Western blot analysis of Arl3 was performed by loading (from left to right): Mouse brain tissue and 231 cell, A375 cell lysates (40µg) on to a 15% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a Arl3 antibody (Product # PA5-50628) at a 1/433 dilution for 2 minutes, followed by a secondary antibody at 1/8000.
Supplier Page from Thermo Fisher Scientific for ARL3 Antibody